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Treatment of Wistar rats for 7 days with 1,3-dipropyl-8-sulfophenylxanthine (DPSPX), an antagonist of adenosine receptors, induces lengthy-lasting hypertension related to marked adjustments in vascular construction and reactivity and renin-angiotensin system activation. This examine aimed at evaluating the function of oxidative stress in the development of DPSPX-induced hypertension and also at figuring out the relative contribution of superoxide radical (O 2 •− ) vs hydrogen peroxide (H 2 O 2 ). Vascular and systemic prooxidant/antioxidant standing was evaluated in sham (saline, i.p., 7 days) and DPSPX (ninety μg/kg/h, i.p., 7 days)-handled rats. Systolic blood stress was decided by invasive and non-invasive strategies. The activity of vascular NADPH oxidase, superoxide dismutase (SOD), catalase and glutathione peroxidase was assayed by fluorometric/spectrophotometric strategies. H 2 O 2 levels were measured using an Amplex Red Hydrogen Peroxide kit. Plasma thiobarbituric acid reactive substances and plasma antioxidant capability had been also measured. As well as we examined the consequences of antioxidants or inhibitors of reactive oxygen species era on blood stress, vascular hyperplasia and oxidative stress parameters. DPSPX-hypertensive rats confirmed elevated exercise of vascular NADPH oxidase, SOD, catalase and glutathione peroxidase, as well as increased H 2 O 2 era. DPSPX-hypertensive rats additionally had increased plasma lipid peroxidation and decreased plasma antioxidant capability. Treatment with apocynin (1.5 mmol/l, per os, 14 days), or with polyethylene glycol (PEG)-catalase (10,000 U/kg/day, i.p., Eight days), prevented the DPSPX-induced effects on blood pressure, vascular construction and H 2 O 2 ranges. Tempol (three mmol/l, per os, 14 days) didn't inhibit these modifications, until PEG-catalase was coadministered.
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發表於 2025-8-9 09:20:04